⚡ RESEARCH REFERENCE GUIDE

Peptide Reconstitution Guide

Step-by-step instructions on reconstituting lyophilized research peptides with Bacteriostatic Water, calculating exact concentrations, and maintaining sample integrity for The PED Shop.

Reconstitution Workflow

Follow this standard operating procedure to prepare research samples safely

1
Equipment Prep

Gather your lyophilized peptide vial, Bacteriostatic Water (BAC water), alcohol prep wipes, and sterile U-100 insulin syringes.

2
Sanitization

Swab the rubber stoppers of both vials thoroughly with alcohol. Equalize air pressure before drawing diluent into syringe.

3
Slow Injection

Inject BAC water slowly down the interior glass wall of the peptide vial. Avoid spraying directly onto the freeze-dried puck.

4
Gentle Dissolution

Gently roll or swirl the vial between your palms until the solution becomes completely clear. Never shake the vial forcefully.

Peptide Dosage & Volume Calculator

Calculate exact U-100 syringe tick marks for any reconstituted peptide dose

Syringe Draw Volume
10 IU
Draw to the 10 mark on a standard U-100 1mL insulin syringe
5,000 mcg/mL
Concentration
0.10 mL
Volume (mL)

Standard Reconstitution Reference Matrix

Common reconstitutions using 2.0 mL Bacteriostatic Water per vial

Peptide Vial BAC Water Added Concentration 10 IU Mark (0.1 mL) Recommended Storage
BPC-157 (5 mg) 2.0 mL 2,500 mcg/mL 250 mcg 2°C – 8°C (Refrigerated)
Retatrutide (10 mg) 2.0 mL 5,000 mcg/mL 500 mcg 2°C – 8°C (Refrigerated)
Tirzepatide (60 mg) 3.0 mL 20,000 mcg/mL 2,000 mcg (2 mg) 2°C – 8°C (Refrigerated)
GHK-Cu (50 mg) 2.5 mL 20,000 mcg/mL 2,000 mcg (2 mg) 2°C – 8°C (Refrigerated)
TB-500 (10 mg) 2.0 mL 5,000 mcg/mL 500 mcg 2°C – 8°C (Refrigerated)
HGH (10 IU Vial) 1.0 mL 10 IU/mL 1.0 IU 2°C – 8°C (Refrigerated)

Storage & Preservation Protocol

Lyophilized Powder: Store freeze-dried vials in a cool, dark location away from direct sunlight. For extended storage (over 3 months), store at -20°C.

Reconstituted Liquid Solution: Once Bacteriostatic Water is added, store the vial refrigerated at 2°C to 8°C (36°F – 46°F). Reconstituted peptide solutions maintain maximum chemical stability for up to 30 to 60 days when refrigerated.